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Tumors were harvested 12 days after initial tumor challenge and dissociated in dissociation buffer rotating at 37C for 30 minutes, passed through a 70 M filter, treated with ACK lysis buffer (ThermoFisher, #A1049201), quenched with RPMI and 10% FBS, and washed in sterile PBS

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The 96-h LC was determined, and significant oxidative damage, enzyme disruption, and tissue lesions were observed under sub-lethal exposure

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