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A potent endogenous antioxidant, glutathione is essential for the livers phase I and phase II detoxification.* However, negative lifestyle issues and adverse conditions that affect liver health can result in a decreased amount of glutathione in the liver

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As a result, it acts close to its source, oxidizing nearby biomolecules, while H 2 O 2 can freely diffuse across cell membranes and oxidize molecules farther away, such as pathogen membrane lipids

Equal cell numbers were collected at various time points, lysed with sample buffer, and examined by immunoblot using an affinity-purified antibody against the C-terminus of GspD (see Methods for details)