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Adding NAD+ back helps reduce senescent cells in dermal fibroblasts [19] and protects against new senescent cells through its antioxidant properties [21]
The slow titration schedule exists specifically to minimize these effects
Levels of the target metabolic substances were calculated from the peak area, by using mass chromatography and indicated as relative amounts following normalization using the internal standard (d-camphor-10-sulfonic acid) peak area
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