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A simple explanation of Methylation blocks: Blocked methylation pathways and Glutathione depletion leads to: Poor detoxification Altered DNA Reduced Immune function Raised inflammation levels Mitochondrial dysfunction All of the above issues are associated with conditions where there is an environmental toxin burden and subsequent increase in infections such as: parasites, bacteria, viruses and fungi such as Candida

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Isolation and Storage of Peripheral Blood Mononuclear Cells, Plasma, and Red Blood Cells Peripheral Blood Mononuclear Cells (PBMCs), plasma, and red blood cells (RBCs) were isolated from whole blood by density gradient centrifugation using ficoll histopaque (Sigma, St