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Injection technique follows these general steps, though specific instructions vary by product: For pens requiring needle attachment: Attach a new pen needle securely, removing both outer and inner needle caps For pens requiring priming: Perform an airshot (flow check) as directed in the product instructions to ensure proper pen function Select the prescribed dose using the dose selector (if applicable) Choose an injection site in the abdomen (at least 2 inches from the navel), front of thigh, or upper arm Cleanse the site with an alcohol swab and allow to air dry Pinch the skin gently and insert the needle at a 90-degree angle Depress the injection button fully and hold for the time specified in your product's instructions (typically 510 seconds) to ensure complete dose delivery Remove the needle, dispose of it immediately in an FDA-cleared sharps container, and do not recap Rotation of injection sites is important to prevent lipohypertrophy, which can impair medication absorption

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Residues involved in the interaction with 5-helix (H387L394) in the receptor of 2 AR (green box) and GLP-1R (purple box) are shown above and below the schematic, respectively