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Briefly, CRM1 was purified from E

The safety data is encouraging: no serious adverse events documented across 100+ preclinical studies, no organ damage, and no deaths even at doses 1,000 times typical use (Sikiric et al., 2014

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For LC-MS, the reaction was prepared with 10 M enzyme, 200 M GTP in 20 mM HEPES buffer, pH 6.8, 150 mM NaCl, 10 mM MgCl 2 .The reaction was carried out at 30 C for 5 min, then deactivated at 95 C for 5 min