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Multiple sequence alignment and phylogenetic assessment For each enzyme family, candidate sequences were aligned using MAFFT 91,92 ( with default parameters and visualised in the multiple sequence alignment editor AliView v1.27 93 ( To provide evolutionary context to the metazoan phylogenetic relationships, we also incorporated non-metazoan sequences

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GLP-1 secreted by the intestine inhibits glucose appearance by inhibiting both nutrient intake and gastric emptying rates while stimulating insulin secretion to facilitate muscle and liver glucose uptake