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However, after exendin-4 stimulation, the receptor association with clathrin-positive regions increased, an effect that correlates with the known rapid GLP-1R internalisation triggered by exendin-4 (31), with this increase however significantly less pronounced for the GLP-1R V229A compared to WT (F/S = 0.16 0.006 and 0.10 0.004 for WT versus V229A, respectively), in agreement with the reduced internalisation propensity displayed by this mutant receptor (Figure 2H)