RRHP 5-hmC Library Prep Kit contact-for-pricing The included Reaction Buffer (4X)

The included Reaction Buffer (4X) contains dNTPs

The Zymo-Spin VI with Zymo Maxi Filter assembly can be used with vacuum manifolds for the purification of DNA or RNA

The methylated DNA standard is purified HCT116 DKO DNA that has been enzymatically methylated at CpG sites

lyophilized

RRHP 5-hmC Library Prep Kit contact-for-pricing The included Reaction Buffer (4X)The RRHP 5 hmC Library Prep Kit is an all inclusive solution for analysis of genome wide 5 hydroxymethylcytosine (5 hmC) positions at single base resolution. The Reduced Representation Hydroxymethylation Profiling (RRHP) method is based on blocking MspI digestion by glucosylating 5 hmC within MspI recognition sites. Fragments lacking glucosylated 5 hmC at the adapter ligation junction will be cleaved and not amplified by PCR. Therefore, only fragments