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Chartoumpekis DV, Ziros PG, Chen JG, Groopman JD, Kensler TW, Sykiotis GP (2019) Broccoli sprout beverage is safe for thyroid hormonal and autoimmune status: Results of a 12-week randomized trial

786-O cells were cultured in RPMI +pyruvate +FBS [RPMI containing 1 mM pyruvate and 25 mM glucose (ThermoFisher, A1049101) supplemented with 10% non-dialyzed FBS and 1% penicillin/streptomycin] at 37C in 5% CO 2

The maximum level of folate biofortification reached in rice seeds exceeds the recommended daily allowance for an adult person (400 g) more than fourfold

Patients should be reviewed 812 weeks after commencing treatment to assess: Symptomatic improvement, particularly resolution of fatigue and neurological symptoms Haematological response (normalisation of haemoglobin and mean cell volume) Reticulocyte count typically rises within one week, indicating bone marrow response Repeat serum B12 testing is generally not useful after starting parenteral therapy, as levels remain elevated for months