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On its own, GHK has moderate biological activity

However, studies using beta-3-adrenergic receptor knockout mice revealed a more complex picture: Long-term AOD-9604 treatment failed to produce weight loss in beta-3-AR knockout mice Acute administration still increased energy expenditure and fat oxidation in knockout mice This suggests beta-3-AR expression enhancement contributes to, but does not directly mediate, lipolytic effects The peptide appears to work through an unidentified receptor mechanism that subsequently influences beta-3-adrenergic signaling pathways

5-Amino-1MQ Combined With a Reduced-Calorie Diet in Mice Later research examined pharmacological NNMT inhibition alongside a diet change

Studies indicate the peptide's antioxidant activity occurs at remarkably low concentrations