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Biologics [e.g., TNF- inhibitors (etanercept), IL-6R blockers (tocilizumab)]: Effective in 60 - 70% of patients but incur prohibitive costs ($20,000 - 50,000 annually), limiting accessibility (only 10 - 20% treated in low-resource settings) (5, 6)

Each coverslip was washed three times with PBS, blocked with blocking solution (1x PBS, 10 % normal goat serum (NGS) and 0.3 % Triton X-100) for 30 min at RT and incubated with primary antibodies (anti-GFP (rabbit polyclonal, used at 1:1000 in IF, Abcam, Cat# ab6556, RRID:AB_305564), anti vGLUT1 (guinea pig polyclonal, used at 1:500 in IF, Synaptic Systems, Cat# 135 304, RRID:AB_887878)) diluted in blocking solution for 2 h at RT

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