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For analysis, blood samples were categorized into three groups: (a) patients with serum samples collected under baseline conditions ( n = 32), (b) patients with serum samples collected at post-seizure ( n = 30), and (c) serum samples from healthy control subjects ( n = 23)

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Follow the specific instructions provided, and consult your doctor or healthcare professional if you have questions about the technique

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Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol

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