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GSH concentration is instead measured by either of two methods: by analysis of GS-NEM conjugates by HPLC in the same sample that is used to measure GSSG or, alternatively, by analysis of GSH by spectrophotometry (GSH recycling method) on one additional sample aliquot that has not been derivatized with NEM

There could be any number of reasons

A Precision Peptide Genetic Test can reveal predispositions in key variants relevant to GLP-1 response, though genetic testing should always be interpreted alongside clinical evaluation and biomarker data