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Despite that UPR helps cells to cope with ER stress, prolonged ER stress induces apoptotic cell death where the accumulation of unfolded protein in ER results in increased expression activating transcription factor 4 (ATF-4) that in turn increases the transcription of the proapoptotic protein, C/EPB-homologous protein-10 (CHOP-10) (Li et al., 2014)

Unlike previous research, which has primarily emphasized the anticancer and specific antibacterial activities of CM-EXOs, this work is the first to position CM-EVs within the context of animal production, comprehensively assessing their stability under simulated digestive conditions, inhibitory efficacy against common veterinary pathogens, free radical scavenging capacity, and regulatory effects on macrophage-mediated inflammatory responses

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