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The cells were rinsed 3 times with 2 mL of PBS buffer under dark condition and then staining with the mitochondrial localization reagent Mitotracker Red (1 M) and incubated under the same culture conditions for 25 min

variabilis , NADPH oxidase activity increased from 45.56 nmol reduced NBT mg -1 protein min -1 in the control to 61.71 nmol reduced NBT mg -1 protein min -1 at 300 g L -1 Pb and to 72.50 nmol reduced NBT mg -1 protein min -1 at 500 g L -1 Pb, corresponding to increases of 35.4% and 59.1%, respectively, relative to the control

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