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Swirling Lightly: In the wake of adding the diluent, tenderly whirl the vial to guarantee exhaustive blending of the powder

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and 2,7-dichlorofluorescin diacetate (DCFH-DA), 2,2-azobis (2-amidinopropane) dihydrochloride (ABAP), cobalt-protoporphyrin IX (CoPPIX), bilirubin and biliverdin were purchased from Sigma-Aldrich (Steinheim, Germany)